FIG. 7.

GCN4 mRNA translation in a gcn2m2 mutant strain (A), a gcn1Δ mutant strain (B), a gcn20Δ mutant strain (B), or the isogenic wild-type strain expressing either the α₁β₁ Na,K-ATPase, α₁(TM4/TM5), or no recombinant protein. Yeast cells carrying the translational reporter GCN4-lacZ were grown and induced with galactose for heterologous expression at time zero, as described in Materials and Methods. β-Galactosidase activities were determined at the indicated time points in purified cytosolic fractions. The isogenic wild-type strain expressing α₁β₁ Na,K-ATPase was included as a control. The results are from at least three independent growth experiments with triple determinations of each data point.