TABLE 2.
Verification of reporter strains used in the present study
| Reporter gene | β-Galactosidase activity (nmol/min mg of protein)a
|
|||
|---|---|---|---|---|
| S288C mutant
|
W303
|
|||
| R | DR | R | DR | |
| GCN4-lacZ | 20 | 270 | 16 | 140 |
| GCN4Δuorf-lacZ | 710 | 680 | ND | ND |
| HIS4-lacZ | 579 | 1726 | ND | ND |
a
Reporter strains were grown in amino acid-supplemented minimal medium to an OD450 of 1, supplemented (derepressed [DR]) or not (repressed [R]) with 50 mM 3-AT, and harvested 24 hours later. β-Galactosidase activities were measured in purified cytosolic preparations. ND, not determined; GCN4-lacZ, a translational fusion between GCN4 at codon 56 and lacZ; GCN4Δuorf-lacZ, identical to the GCN4-lacZ fusion except that the initiation codons of the four upstream open reading frames were altered to GTG; HIS4-lacZ, a translational fusion between codon 10 of HIS4 and codon 8 of lacZ.