FIG. 3.

Developmental changes caused by ΔphnA mutation. (A) Enhanced Hülle cell formation on solid MM and development of conidiophores (CP) in liquid submerged culture caused by ΔphnA mutation. Whereas the wild type (WT; RJA56.25) did not produce conidiophores even at 30 h, the ΔphnA (TJAP3) and ΔsfaD (RSRB1.15) mutant strains began to elaborate conidiophores as early as 18 h in liquid YM. The colony and microscopic photographs shown were taken after 4 days on solid MM and after 22 h in liquid YM, respectively. (B) Northern blot analysis of brlA in various mutants. While no brlA mRNA was detected during the vegetative growth stage of the WT (RJA56.25) or ΔgpgA (RJAG19.9) strain, a precocious and elevated accumulation of brlA mRNA was evident for the ΔphnA, ΔsfaD, and ΔsfaD ΔgpgA mutant strains (TJAP3, RSRB1.15, and RJA55.4). Equal loading of total RNAs was evaluated by ethidium bromide staining of rRNA.