TABLE 2.

Oligonucleotides used for this study

Primer	Sequence	Position and/or purpose
OJA6	cg G GAT CCg cat tgt acg ata atc ttg	Forward 5′ flanking region of phnA ORF for phnA deletion with BamHI tail
OJA7	cg G GAT CCa gac gtc cat ttt gca att c	Reverse 3′ flanking region of phnA ORF for phnA deletion with BamHI tail
OJA8	ccg CTC GAG agg agg cct gta gtt gg	Reverse 5′ flanking region of phnA ORF for phnA deletion with XhoI tail
OJA9	ccg CTC GAG tga cat caa cat aac tct cc	Forward 3′ flanking region of phnA ORF for phnA deletion with XhoI tail
OJA10	gcg cag act gat tca gca tgg	5′ Forward region of phnA; confirmation of ΔphnA mutation
OJA202	gaa gct cac aac atg ttg	Sequencing of phnA; confirmation of ΔphnA mutation
OJA232	agc gca agt tcg atg ata tgg acc	5′ Forward region of metG
OJA233	aag atg tga tcg atg agg cat	3′ Reverse region of metG
OJA234	cga agc cat cca gaa ggt gat	5′ Forward region of flbA; confirmation of ΔflbA mutation
OJA236	ggt cca tat cat cga act tgc gct a gaa gag tgc agg tcg gag tgg	5′ Flanking region of flbA with metG tail (underlined)
OJA237	atg cct cat cga tca cat ctt a gct cac aac gtt cat gac agt aat	3′ Flanking region of flbA with metG tail (underlined)
OJA238	tcg tgt cag tca ctt gaa tga	3′ Reverse region of flbA
OJA235	cta gca tag cac cat acc cat	5′ Nested primer for flbA deletion
OJA239	gga tgg tag gta tgt gaa tca	3′ Nested primer for flbA deletion
OJA240	ttc gtc atc ccg tac tgt att	5′ Forward region of flbA
OJA241	gtg cct aga aag taa tat cga	3′ Reverse region of flbA; confirmation of ΔflbA mutation
OJA144	ttc cta tgt cat ggt agc	5′ Forward region of stcU
OJA145	gta cgg tgt gac taa ctg	3′ Reverse region of stcU
OJA242	gct gtc gat ctt tgt acc ctg	5′ Forward region of laeA
OJA243	cgt tcc tgg atg tgg tcg cct	3′ Reverse region of laeA
OJA244	cct gta tca tat gca aat atc	5′ Forward region of aflR
OJA245	atc att cca gat tat tca acc	3′ Reverse region of aflR