FIG. 2.

Effect of ebs1-1 mutation on accumulation of NMD-sensitive transcripts. (A) Northern blotting was used to determine the CYH2 pre-mRNA/mRNA ratio in an ebs1-1 strain transformed with an empty vector or a CEN or 2μm plasmid carrying EBS1. The amount of change (fold) in the CYH2 pre-mRNA/mRNA ratio is indicated, along with the standard error from three experiments (in parentheses). The relative accumulations of his4-38 mRNA (B) and leu2-2 mRNA (C) were determined in an ebs1-1 mutant strain transformed with the plasmids indicated above. The signals were normalized to the PGK1 and HIS4 mRNAs, respectively.