FIG. 4.

Effects of EBS1 expression levels on translation. (A) Relative levels normalized to SCR1 RNA were determined for transformants carrying an empty vector or a CEN or 2μm plasmid carrying EBS1. Enzyme activities were adjusted relative to lacZ mRNA levels to compare relative rates of translation per transcript. (B) β-Galactosidase-to-luciferase enzyme activity ratios were calculated for transformants carrying dual reporters that contain an internal stop codon and either an empty vector or a CEN or 2 μm plasmid carrying EBS1. (C) GFP fluorescence in cells expressing Ebs1-GFP compared to DAPI (4′,6′-diamidino-2-phenylindole) staining of DNA. Bar = 5 μm.