FIG. 3.

Minimal region in ¹Cuf1⁶¹ required for translocation to the nucleus. The left panel shows schematic illustration of ¹Cuf1⁶¹ and its N-terminal and C-terminal deletions fused to tandemly repeated copies of the GFP coding sequence (GFP_2x). The middle panel represents subcellular locations of the fusion proteins determined by fluorescence microscopy. The right panel represents cells viewed by Nomarski microscopy to examine cell morphology. A single copy of the GFP protein (GFP_1x) was expressed from the cuf1⁺ promoter as a control for GFP localization. Cells were grown in EMM containing 100 μM BCS.