FIG. 3.

Pheromone response after deletion or overexpression of RGS proteins. (A) Wild-type (WT) cells and the indicated mutant cells were transformed with a plasmid containing the pheromone-inducible FUS1 promoter and lacZ reporter gene (pRS423-FUS1-lacZ), grown to mid-log phase, and treated with the indicated concentration of α-factor for 90 min. β-Galactosidase activity was measured spectrofluorometrically and expressed as arbitrary fluorescence units. (B) Wild-type cells that overexpress the indicated RGS protein (in plasmids pAB27 or pAB23), cotransformed with the FUS1-lacZ reporter, were assayed for β-galactosidase activity as described above. (C) Transcription reporter assay control experiments. Wild-type cells and the indicated gene deletion mutants were assayed for β-galactosidase activity as described above. Data shown are typical of three independent experiments performed in triplicate. Error bars indicate standard errors of the means (SEM).