TABLE 1.
Effects of RGS protein expression on mating efficiencya
| Strain | Plasmid | Mating % (SEM %) |
|---|---|---|
| WT | 100 (4.4) | |
| sst2Δ | 55 (7) | |
| rgs2Δ | 120 (1.1) | |
| rax1Δ | 130 (1.5) | |
| mdm1Δ | 120 (9) | |
| WT | pAB | 100 (6) |
| WT | pAB-SST2 | 86 (10) |
| WT | pAB-RGS2 | 90 (5) |
| WT | pAB-RAX1 | 110 (8) |
| WT | pAB-MDM1 | 90 (7) |
a
Mating, mating efficiency was determined as the mating frequency of the mutant divided by the mating frequency of the WT control; control was defined as 100%. SEM is expressed as a percentage. Data are representative of assays performed three times in triplicate.