TABLE 3.
Group III mutants are pheromone-unresponsivea
| Gene (strain) | ID? | Role |
|---|---|---|
| STE2 (YFL026W) | YES | α-factor receptor |
| STE3 (YKL178C) | NA | a-factor receptor |
| STE4 (YOR212W) | YES | G protein β subunit |
| STE5 (YDR103W) | YES | Kinase scaffold |
| STE6 (YKL209C) | NT | a-factor export |
| STE7 (YDL159W) | YES | MAP kinase kinase |
| STE8/SIR3 (YLR442C) | YES | Transcription silencing |
| STE9/SIR4 (YDR227W) | YES | Transcription silencing |
| STE11 (YLR362W) | YES | MAP kinase kinase kinase |
| STE12 (YHR084W) | NT | Transcription factor |
| STE13 (YOR219C) | NA | α-factor processing |
| STE14 (YDR410C) | ND | a-factor processing |
| STE16/RAM1 (YDL090C) | NA | a-factor processing |
| STE18 (YJR086W) | NT | G protein γ subunit |
| STE20 (YHL007C) | ND | MAP kinase kinase kinase |
| STE21/MSN5 (YDR335W) | NO | Nuclear import/export |
| STE22/AXL1 (YPR122W) | NA | a-factor processing |
| STE23 (YLR389C) | NA | a-factor processing |
| STE24 (YJR117W) | YES | a-factor processing |
| STE50 (YCL032W) | YES | Ste11 regulator |
| SIR2 (YDL042C) | YES | Transcription silencing |
a
List of genes known to be required for pheromone signaling were identified (“ID”) by α-factor screening (“YES”) or unidentified because they are required for a-factor responsiveness or for pheromone processing and are not applicable (NA) or unidentified because they were absent from the collection and not tested (NT) or strains containing them failed to grow and so they were not done (ND). Deletion of STE21 results in partial sterility (http://www.yeastgenome.org).