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Effect of nontemplate strand trinucleotide substitutions on Rho-mediated RNA release. (A) Rho-mediated release of RNA was measured from complexes stopped on nontemplate heteroduplex DNAs made with the substitutions shown. Black bars, release compared with wild-type homoduplex DNA; gray bars, release compared with mutant homoduplex DNA. (B) The time course of RNA release from complexes on wild-type homoduplex DNA.
