FIG. 1.
M3 inhibition of CCL19- and CCL21-induced chemotaxis. (A) Flow cytometry of nontransfected Ba/F3 cells (open curve), Ba/F3 stably expressing human CCR7 (filled curve), and IgG control (dashed line) using an hCCR7-specific antibody. (B) Chemotaxis of Ba/F3-hCCR7 cells toward CCL19 (▴) and CCL21 (▪) and nontransfected Ba/F3 cells toward CCL19 (▾) and CCL21 (♦). (C) CCL21 (▪) and CCL19 (▴) (each at 2.0 nM) was preincubated with the indicated concentrations of rM3 for 30 min prior to the chemotaxis assay. Data are the means and standard deviations (error bars) of triplicates. The results shown are representative of three independent experiments.