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. 2003 Jan;77(1):291–300. doi: 10.1128/JVI.77.1.291-300.2003

FIG. 2.

FIG. 2.

Selective inhibition of HIV-1-specific cytolytic activity by CMA but not anti-FasL or anti-TNF-α antagonistic antibodies. Blood and mucosal CD8+ clones were pretreated with CMA (1 to 100 nM), anti-FasL MAb (12 to 50 μg/ml), anti-TNF-α MAb (5 to 20 μg/ml), or control mouse immunoglobulin G for 2 h. In some experiments, a combination of two or three blocking agents at the highest (experiment 1) or lowest (experiment 2) concentrations was used. The figure depicts cytolysis of CD8+ clones isolated from patients CC0710 (blood and cervix), NP002 (rectum), and NP014 (semen). Blood and cervical clones from patient CC0710 recognized HIV-1 Gag within aa 264 to 272. Blood (data not shown) and semen HIV-specific CTL clones from patient NP014 recognized HIV-1 Gag within aa 164 to 172. Blood (data not shown) and rectal T-cell clones from patient NP002 recognized HIV-1 Gag within aa 269 to 277. Ab, antibody.