TABLE 1.
Characteristics of the 12 HIV-1-infected study participants and the CD8+ CTL clones derived from blood and mucosa
| Study participant (HLA type) | Gender, duration of infection (yr) | CD4+ T cell count (cells/μl) | Amt. of HIV-1 RNA (no. of copies/ml) in:
|
No. of HIV-1-specific CD8+ CTL clones in:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Blood
|
Mucosa
|
|||||||||||
| Blood | Mucosaa | Env | Gag | Pol | Total | Env | Gag | Pol | Total | |||
| MP 0471 (A24; B27, 62) | Male, 4 | 443 | 2,500 | 2,226 | 2 | 29 | 9 | 40 | 6 | 5 | 3 | 14 (semen) |
| CC 0710 (A24, 33; B14, 27) | Female, 9 | 500 | 3,000 | 10,000 | 7 | 15 | 31 | 53 | 0 | 10 | 3 | 13 (cervix) |
| NS 0909 (A31; B7, 57) | Female, 5 | 1,000 | 10,000 | 8,000 | 31 | 77 | 16 | 124 | 2 | 31 | 5 | 38 (cervix) |
| CHPIC 1013 (A3, 28; B35, 60) | Male, 4 | 336 | 18,750 | 868 | 1 | 3 | 5 | 9 | 0 | 2 | 3 | 5 (semen) |
| NP 001 (A30, 68; B57) | Male, 14 | 1,145 | <200 | NAb | 1 | 5 | 0 | 6 | 1 | 6 | 0 | 7 (rectum) |
| NP 002 (A2, 24; B27, 62) | Male, 13 | 716 | 414 | <400 | 0 | 34 | 11 | 45 | 0, 0 | 2, 8 | 0, 12 | 22 (semen, rectum) |
| NP 005 (A1, 66; B52, 57) | Male, 13 | 968 | 75 | NA | 0 | 47 | 0 | 47 | 0 | 17 | 0 | 17 (rectum) |
| NP 007 (A2, 3; B7, 13) | Male, 12 | 867 | 55 | 52,881 | 1 | 9 | 1 | 11 | 0 | 4 | 2 | 6 (rectum) |
| NP 012 (A2, 11; B44, 50) | Male, 14 | 590 | <200 | <400 | 0 | 2 | 1 | 3 | 0 | 2 | 1 | 3 (rectum) |
| NP 013 (A3, 31; B27, 38) | Male, 11 | 478 | <200 | <400 | 0 | 13 | 1 | 14 | 0 | 5 | 0 | 5 (rectum) |
| NP 014 (A3, 28; B53, 57) | Male, 13 | 1,129 | <200 | <400 | 3 | 51 | 13 | 67 | 1 | 7 | 11 | 19 (semen) |
| NP 015 (A2, 32; B27, 62) | Male, 15 | 666 | 5,857 | <400 | 1 | 24 | 37 | 62 | 0, 1 | 14, 3 | 0, 2 | 20 (semen, rectum) |
| Total (n = 12) | 10/2 (M/F), 12.5 (median) | 691 (median) | 307 (median) | 534 (median) | 47 (10%) | 309 (64%) | 125 (26%) | 481 | 11 (7%) | 116 (69%) | 42 (25%) | 169 |
a
Mucosal HIV-1 RNA measurements were performed with seminal plasma (male volunteers) or cervical Sno-strip (female volunteers) as described in Materials and Methods.
b
NA, not applicable.