FIG. 3.

Chemical and enzymatic probing of plussense DI-70F transcripts. (A) DI-70F RNA was treated in vitro with RNase V₁, DEPC, or CMCT, and the products were subsequently analyzed by primer extension. Primer extension products generated were separated in an 8% sequencing gel. Also indicated are lanes showing untreated control transcripts extended with PR47 (control) and DI-70F plasmid DNA sequenced with PR47. (B) Optimal computer-predicted secondary-structure model of region III plus strand that includes a summary of enzymatic and chemical modifications (RNase V₁, DEPC, or CMCT). Stronger and weaker relative reactivities are indicated by larger and smaller symbols, respectively.