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. 2003 Jan;77(1):754–761. doi: 10.1128/JVI.77.1.754-761.2003

FIG. 2.

FIG. 2.

Western analyses of the proteins expressed by various gag-pol expression constructs in transfected cells and cell-free virions. (A and B) Western analyses of viral proteins in cell-free virions. Results from virions produced by cells transfected with MLV- and SNV-based constructs are shown in panels A and B, respectively. Equal numbers of stably transfected cells were plated; cell-free supernatants were harvested 48 h later. An equal portion of each sample was loaded in the lanes marked 1, whereas one-third and one-ninth of the portions were loaded in lanes marked 1/3 and 1/9, respectively. (C and D) Western analyses of cell lysates generated from transfected E1 cells.Results from MLV- and SNV-based constructs are shown in panels C and D, respectively. Cell lysates from E1 cells transfected with only pBSpac were used as controls. A rabbit anti-MLV CA antibody was used to probe the Western blots shown in panels A and C, whereas a rabbit anti-SNV CA antibody was used to probe the Western blots shown in panels B and D. Anti-MLV and -SNV CA antibodies were kind gifts from the AIDS Vaccine Program, Science Applications International Corporation, and Nancy Rice, NCI-Frederick, respectively.