TABLE 2.
Comparison of adjusted viral titers generated by various gag-pol expression constructs
| Expt |
gag-pol expression construct titer
|
|||||
|---|---|---|---|---|---|---|
| MLV derived (104 CFU/ml)a,b
|
SNV derived (103 CFU/ml)a
|
|||||
| pWZH30 | pMgpSnb | pMgpScb | pRD136 | pSgpMnb | pSgpMcb | |
| 1 | 4.2 | 1.3 | 0.84 | 0.45 | 0.08 | 0.06 |
| 2 | 7.2 | 4.4 | 4.0 | 1.4 | 0.36 | 0.04 |
| 3 | 2.6 | 0.65 | 0.48 | 2.1 | 0.65 | 0.25 |
| 4 | ND | ND | ND | 0.83 | 0.18 | 0.08 |
| 5 | ND | ND | ND | 2.0 | 0.43 | 0.18 |
| Mean ± SE | 4.7 ± 1.3 | 2.1 ± 1.2 | 1.8 ± 1.1 | 1.4 ± 0.3 | 0.34 ± 0.1 | 0.12 ± 0.04 |
a
Viral titers were standardized to the RT activities of the virions containing the appropriate wild-type viral proteins.
b
ND, not determined.