FIG. 2.

PCR and agarose gel electrophoresis analysis of recombinant viral DNAs. Primers 1 and 2 were designed to amplify the gp64 promoter region from each virus (lanes 1 to 5), whereas primers 3 and 4 were designed to amplify the entire gp64 gene, including the promoter and open reading frame, from each virus (lanes 6 to 10). The products obtained with primers 3 and 4 were digested with Bsu36I prior to electrophoresis to distinguish between the parental (Ac64DCHspBlue) and recombinant viruses. The drawing below the gel shows the approximate locations of the primer sequences (numbered arrows), pro gene, gp64 open reading frame (ORF), and Bsu36I (Bsu) sites in the parental virus. Lanes 1 and 10, no DNA; lane 2, AcMNPV; lanes 3 and 7, AcCtl-64HB; lanes 4 and 8, AcCtlNt-64HB; lanes 5 and 9, AcLate21/20-64HB, lane 6, Ac64DCHspBlue. The positions of molecular size markers (in kilobases) are indicated to the left of the blot.