FIG. 2.

Localization of EAV M and GP₅ in BHK-21 cells transfected with wt EAV and a selection of mutants. Cells were fixed at 8 to 10 h posttransfection and processed for a double IFA using an anti-M rabbit serum, an anti-GP₅ mouse MAb, and appropriate fluorescent conjugates. (A1 to E1) Labeling for M; (A2 to E2) signal for GP₅. Constructs: A, pA45 (wt); B, pA45/C8S (M mutant); C, pA45/C34S (GP₅ mutant); D, pA45-80.4 (pseudorevertant); E, pA45-80.4/C34S (pseudorevertant with the Cys-34- to-Ser mutation in GP₅). (A and D) Transport of both M and GP₅ to the Golgi complex and the accumulation of an excess of M in the ER (18). Transport of pA45-80.4 GP₅ (D2) seems to be less efficient than for wt EAV (A2); see the text for details. For the Cys mutants in panels B, C, and E, transport to the Golgi complex was not observed.