FIG. 3.

(A) Schematic representation of the structure of the GP₅ ectodomain for wt EAV (pA45), the GP₅ Cys-80-to-Ser mutant (pA45/C80S), and pseudorevertant 80.4 (pA45-80.4). The putative GP₅ signal sequence (ss) and transmembrane domain (TM) are depicted in gray. Cys residues and the single GP₅ N-glycosylation site (Asn-56) are indicated. (B) Example of a plaque assay of the virus harvested (24 h postinfection) from BHK-21 cells infected with wt EAV (left; dilution, 10⁻⁷) or pseudorevertant A45-80.4 (right; dilution, 10⁻⁵). The latter virus consistently produced 20- to 100-fold-reduced infectivity titers and somewhat smaller plaques.