TABLE 2.
Composition of the GP5 and M proteins of the EAV constructs
| Construct | Mutated protein | Mutation(s) | Phenotype |
|---|---|---|---|
| pA45 | NAa | NA | Wild type |
| pA45/C8S | M | Cys-8 → Ser | No infectivity |
| pA45/C34S | GP5 | Cys-34 → Ser | No infectivity |
| pA45/C57S | GP5 | Cys-57 → Ser | No infectivity |
| pA45/C63S | GP5 | Cys-63 → Ser | No infectivity |
| pA45/C66S | GP5 | Cys-66 → Ser | No infectivity |
| pA45/C80S | GP5 | Cys-80 → Ser | No infectivity |
| pA45/QCS | GP5 | Asn-56 → Gln | No infectivity |
| pA45/GCS | GP5 | Asn-56 → Gly | No infectivity |
| pA45/SCS | GP5 | Asn-56 → Ser | No infectivity |
| pA45/NCG | GP5 | Ser-58 → Gly | No infectivity |
| pA45-80.4 | GP5 | Thr-52 to Asp-79 replaced by Asn; Cys-80 → Ser | Attenuated |
| pA45-80.4/C34S | GP5 | Thr-52 to Asp-79 replaced by Asn; Cys-80 → Ser; Cys-34 → Ser | No infectivity |
a
NA, not applicable.