FIG. 7.

HSV-1-encoded protein recognized by anti-HSV antibodies. Lysates from metabolically labeled cells infected with wild-type HSV-1 were immunoprecipitated with 1 mg of hIgG (lane 1 from left), 40 μg of rabbit anti-HSV IgG (lane 2), 40 μg of rabbit nonimmune IgG (lane 3), 10 μg of anti-gE (lane 4), 70 μg of anti-gI (lane 5), 10 μg of anti-gB (lane 6), or 2.5 μl of a control antibody, AU5 ascites fluid (Covance Scientific) (lane 7), that is not specific for any HSV glycoprotein. Immunoprecipitated proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the gels were dried, and labeled proteins were visualized by phosphorimaging analyses.