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Zinc-binding activity of C2 protein. Zinc-affinity pull-down assays were performed in buffers containing 50 mM NaCl (A), 100 mM NaCl (B), and 200 mM NaCl (C) with protein extracts from Sf9 cells infected with wild-type baculovirus (lane 1) and recombinant baculovirus expressing His-tagged C2 (lane 2), C2-C46I (lane 3), C2-C38N (lane 4), and C2-C36R (lane 5). His-tagged C2 protein with the predicted size was detected using a monoclonal antibody raised against the histidine tag.
