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DNA-binding activity of C2 protein. Southwestern assays were performed using dsDNA (A and B) and ssDNA (C and D) in buffers containing 300 mM KCl (A and C) and 600 mM KCl (B and D). Assays contained lysozyme (lanes 1), extracts from E. coli expressing maize streak virus coat protein (lanes 2), C2-C46I (lanes 3), C2-C38N (lanes 4), C2-C36R (lanes 5), and C2 (lanes 6), and extracts from E. coli transformed with expression vector pEHISTEV alone (lanes 7).
