FIG. 2.

Decreased expression of B-cell developmental transcription factors in B cells from LMP2A transgenic mice. (A) Semiquantitative RT-PCR was performed with total RNA from CD19⁺ bone marrow (BM) (left) and splenic (SP) (right) B cells from wild-type (WT) and LMP2A transgenic mice. Primers specific for E47 (E2A), EBF, and Pax-5 were utilized, along with GAPDH as a control for RNA levels. Each experiment was repeated three times, and a representative gel is shown. (B) The levels of expression of E47, EBF, and Pax-5 were normalized to the levels of GAPDH, and the amount of expression in wild-type B cells was set to 100%. Error bars indicate standard deviations.