FIG. 5.

E2A activity is inhibited in B cells from LMP2A transgenic mice. DNA binding assays were performed with nuclear extracts prepared from bone marrow (BM) and splenic (SP) B cells from wild-type (WT) and LMP2A transgenic mice. Nuclear extracts were examined for binding to a biotinylated κE2 DNA probe in order to detect E2A-specific DNA binding activity. Bound protein was selected by incubating with streptavidin-coated magnetic beads followed by purification on magnetic columns as described in Materials and Methods. Western blotting was then performed with antibodies specific for E2A (upper gel). Duplicate SDS-polyacrylamide gels were run, and DNA-bound E2A was identified by silver staining (lower gel). Each experiment was repeated two times, and a representative gel is shown.