TABLE 1.
Expression of B-cell-specific genes in DNA microarrays
| Gene | Fold changea
|
|
|---|---|---|
| BM | Spleen | |
| Transcription factor genes | ||
| E2A | −2.2 ± 0.2 | −2.3 ± 0.2 |
| EBF | −3.5 ± 1.0 | −2.2 ± 0.3 |
| Pax-5 | −2.1 ± 0.2 | −2.6 ± 0.3 |
| Id2 | 1.7 ± 0.2 | 3.9 ± 0.1 |
| SCL | 1.4 ± 0.3 | 2.3 ± 0.1 |
| PU.1 | −3 ± 0.1 | −1.5 ± 0.3 |
| Spi-B | −1.7 ± 0.2 | −2.3 ± 0.2 |
| LEF-1 | −3.2 ± 0.2 | −1.5 ± 0.2 |
| TCF-1 | 2.6 ± 0.3 | 1.2 ± 0.6 |
| B-cell-specific gene | ||
| Ig-α (mb-1) | −2.1 ± 0.1 | −2.2 ± 0.2 |
| Ig-β (B29) | −1.5 ± 0.3 | −1.6 ± 0.2 |
| RAG-1 | −1.9 ± 0.3 | 1.8 ± 0.2 |
| RAG-2 | −2.8 ± 0.3 | 1.6 ± 0.5 |
| Vpre-B | −2.6 ± 0.2 | −2.4 ± 0.1 |
| Lambda 5 | −2.7 ± 0.5 | −1.3 ± 0.3 |
| TdT | −3.6 ± 0.2 | −1 ± 0.8 |
| J chain | 4.3 ± 0.5 | 6.7 ± 2.1 |
a
Identical microarray experiments were performed in duplicate with CD19+ bone marrow (BM) and splenic B cells from wild-type and LMP2A transgenic mice. The average fold change in gene expression in cells from LMP2A transgenic mice versus wild-type mice from the two experiments is shown, along with the standard deviation. Negative values indicate decreased expression.