FIG. 7.

Deletion of the C-terminal conserved region abolishes nuclear punctate localization of GFP. The three deletion mutants of VP26 were fused to GFP, and these constructs were transfected into RPE-1 cells. The wild-type control was also transfected into cells. The next day the cells were infected with KΔ26Z, which provides the remaining capsid proteins. Following infection for 16 h, the live infected cell cultures were visualized in a fluorescence microscope (40× objective).