FIG. 7.

Transport of gK to cell surfaces and gK-mediated cell fusion require additional viral functions. Vero cells were transfected with plasmids expressing gKV5DI (A1 to A3), gKV5DIII (B1 to B3), gKsyn20V5DI (D1 to D3), and gDV5 (C1 to C3). At 16 hpt, cells were either mock infected (A1, B1, C1, D1, A2, B2, C2, and D2) or infected with ΔgK (A3, B3, C3, and D3). At 12 hpi, infected cells were immunohistochemically processed under either live (A1, B1, C1, D1, A3, B3, C3, and D3) or fixed and permeabilized (A2, B2, C2, and D2) conditions with anti-V5 antibody (A1 to A3, B1 to B3, and D1 to D3) or anti-gD (C1 to C3).