FIG. 8.

Coexpression of the UL20 protein enables transport of gK to cell surfaces but not gK-mediated cell-to-cell fusion. Vero cells were transfected with either individual plasmids or combinations of plasmids. Transfections with individual plasmids were as follows: pCMV-gKV5DI (A1), pCMVgKsyn20V5DI (B1), pCDNA3-gB/gD (E), and pCMVUL20 (C). Transfections with combinations of plasmids were as follows: pCMV-gKV5DI + pCMVUL20 (A2), pCMVgKsyn20V5DI + pCMVUL20 (B2), pCMV-gKV5DIII + pCMVUL20 (D), pCDNA3-gB/gD + pCMV-gKV5DI (A3), and pCDNA3-gB/gD + pCMVgKsyn20V5DI (B3). Cell surfaces of transfected cells were stained under live conditions with anti-V5 antibody for detection of gK, except for the cells shown in panel E, which were stained with anti-gD antibody for the detection of gD.