FIG. 9.

Experimentally validated model of HSV-1 gK membrane topology. The originally predicted four hpd's of gK (8) were adjusted according to computer-based predictions and are shown as embedded within the lipid bilayer. The locations of the sites where the four V5 tags were inserted and the experimentally derived topology of each tagged domain are as shown. The schematic includes demarcations for the signal peptide and its cleavage site, the two N-linked glycosylation sites within domain I, and the location of syncytial mutations.