FIG. 4.

Epitope maps of the unique effects of alanine substitutions on MAb binding affinity. Color scheme and labeling are as described in the legend to Fig. 3, except that amino acid mutations that did not significantly affect antibody binding are also indicated (colored black). (Top panels) Differential maps of alanine point mutations for which the effect on MAb binding was unique compared to the effects on the binding of the other two MAbs. (Bottom panels) Differential maps of the unique effects of alanine substitutions on binding by MAbs b3 and b6 in comparison to MAb b12.