FIG. 5.

Alanine mutants that were selected for neutralization assays. Labeled amino acids colored grey indicate mutant pseudovirions for which there was a reasonable correlation between b12 binding to monomeric gp120 and neutralization efficiency. Residues labeled and colored yellow indicate pseudovirions which were neutralized equally as well as or better than wild-type virus despite a decrease in the b12 affinity for monomeric gp120 of the respective mutant. Residues labeled and colored red indicate pseudovirions which were not neutralized as well as the wild-type virus despite an increase in the b12 affinity for monomeric gp120 of the respective mutant.