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. 2003 Jan;77(1):340–352. doi: 10.1128/JVI.77.1.340-352.2003

FIG. 1.

FIG. 1.

Synthesis of 5′-terminal sgRNA1 by the 5′TR. (A) Schematic representation of the organization of the CTV replicon, CTV-ΔCla333, and CTV replicons containing portions of CTV, nt 524 to 956 (CTV:524/956), nt 524 to 1164 (CTV:524/1164) or nt 1497 to 2124 (CTV:1497/2124) placed between the end of ORF 1a/1b and the truncated p23 ORF. Nucleotide numbers of the 5′ regions (solid black lines) ligated into CTV-ΔCla333 are also shown. The open boxes represent ORFs 1a and 1b and the truncated p23 ORF. The two papain-like proteases (PRO), methyltransferase-like (MT), helicase-like (HEL), and RNA-dependent RNA polymerase (RdRp) domains of ORF 1a/1b in the genome of CTV-ΔCla333 are indicated. The relative positions of the 5′TR and non-5′TR elements (black boxes with one or two asterisks, respectively) in CTV-ΔCla333 are indicated below the L-ProI domain. (B) Northern blot analysis of the total RNA isolated 3 and 4 days postinoculation (dpi) of N. benthamiana mesophyll protoplasts and hybridized with the 5′ plus- and minus-strand (left and right panels, respectively) RNA-specific probes. Positions of the genomic (g), 5′-terminal sgRNA1, and LMT1 RNAs are indicated.