FIG. 3.

Synthesis of a 3′-terminal sgRNA from the 5′TR element in a dRNA. (A) Schematic diagram of the CTV dRNA generated by a large internal deletion between nt 2892 and 18394 in CTV9. The different domains of ORF 1a/1b are as outlined in the legend to Fig. 1A. (B) Northern blot hybridizations showing the accumulation of the 3′ and 5′ plus- and minus-strand RNAs from the total RNA isolated from protoplasts transfected with the helper replicon (CTV-ΔCla333) (lanes 1) and the helper replicon plus the dRNA (lanes 2). The positions of the genomic (g) RNA of the helper replicon (arrowheads) and the dRNA (arrows) are indicated. The 3′-terminal sgRNA (indicated by an asterisk, observed only with the 3′ plus-strand RNA-specific probe) and LMT1 RNAs (observed only with the 5′ plus-strand RNA-specific probe) are also indicated.