FIG. 4.

Deletion analysis of the 5′TR in the 3′ position in CTV-ΔCla333. (A) Schematic diagram of CTV-ΔCla333 containing progressive deletions from the 5′ and 3′ ends in the 5′TR with their corresponding nucleotide endpoints. (B) Results of the accumulation of 3′ plus- and 3′ minus-strand (left and right panels, respectively) genomic (g) and sgRNA in Northern blot hybridization analysis of the total RNA isolated 3 and 4 days postinoculation (dpi) of the protoplasts transfected with the mutants outlined in panel A. Positions of the genomic and sgRNAs (sg) are indicated. In the bottom panels, only the top portion of the Northern blot hybridizations is presented, showing the accumulation of RNAs that reacted with 5′-terminal plus-strand RNA-specific (left) or 5′-terminal minus-strand-specific (right) riboprobes.