FIG. 8.

Replication of full-length CTV containing mutations in the 5′TR. A diagrammatic representation of mutations in CTV is shown. A Northern blot hybridization analysis of the total RNA isolated 4 days after inoculation of protoplasts inoculated with the wild type (CTV9) or mutants CTV9-M13 to CTV9-M16 (mutations in the 5′TR of CTV9-M13 to CTV9-M16 are as in CTV-ΔCla M13 to CTV-ΔClaM16 in Fig. 7A, respectively) and probed with the 3′ plus-strand RNA-specific riboprobe. Positions of the genomic (g) and sgRNAs are indicated.