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Effect of the mutagenized DNA curvature on in vivo transcription of K-81 psbA2. Recombinant S.elongatus PCC 7942 cells, which carry the K-81 psbA2 5′-upstream region from –237 to +46 containing the CIT, were cultivated under continuous white light, then entrained into a cycle of L/D/L or L/HL/L. Total RNA was isolated and primer extension was performed using the lacZ-R1 primer (7). The signal intensity on X-ray film (left) is shown as a relative value (right).
