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. 2003 Jan;23(1):414–423. doi: 10.1128/MCB.23.1.414-423.2003

FIG. 3.

FIG. 3.

DP103 harbors a repression domain at aa 721 to 825. (A) CV-1 cells were cotransfected with increasing amounts (0, 0.03, 0.1, 0.3, 1.0, and 3.0 μg) of either full-length DP103 or DP103 fragments, fused to GAL4, along with the reporter plasmid GAL4 × 5-tkLuc. Results (means ± SD) are expressed as cRLU normalized to β-galactosidase activity and represent three independent experiments, each performed in duplicate. (B) Western immunoblotting of the GAL4-DP103 fusion proteins analyzed in panel A, demonstrating equal cellular expression of transfected plasmids. Lysates (30 μg) were prepared from CV-1 cells that were transfected with 3 μg of each DP103 plasmid, separated by SDS-PAGE, and immunodetected by using anti-GAL4 antibody as described in Materials and Methods. (C) The DEAD-box protein p68 (3 μg, expressed as chimeric protein GAL4-p68) does not repress the reporter plasmid GAL4 × 5-tkLuc in CV-1 cells. Results are means of three independent experiments, each performed in duplicate, and are expressed as fold over control in which the empty GAL4 plasmid was used.