FIG. 7.

Smp1 nuclear localization depends on Hog1. The wild-type and the hog1Δ mutant strains were transformed with a plasmid carrying a GFP-tagged Smp1 expressed under its own promoter. The cells were grown and Smp1-GFP was detected by fluorescence microscopy as described in Materials and Methods. (A) Smp1 localizes into the nuclei of wild-type cells in the stationary phase. Cells of the indicated types carrying Smp1-GFP were grown to the stationary phase, and images were taken. Representative images showing the localization of Smp1-GFP are presented. The positions of the nuclei were determined by DAPI staining. (B) Quantitative data were obtained from wild-type or hog1Δ cells growing at the mid-logarithmic (Mid log.) or stationary phase of growth. The data represent the results of three independent experiments which included counting 300 cells each.