FIG. 8.

Hog1 and Smp1 are required for cell viability in stationary phase. Wild-type (•), hog1Δ (▿), smp1Δ (○), or hog1Δ smp1Δ (▾) cells were grown in minimal medium to stationary phase and allowed to grow for the indicated period of time. Viability was determined by adding PI and measuring the percentage of PI-positive cells by using a flow cytometer. The results are the measurements in duplicate from four independent experiments. The data were confirmed by Phloxin B staining and visual microscopy (see Materials and Methods).