FIG. 8.

Inhibition of RAR transactivation by HALR-SET. (A) The retinoid-responsive β-RARE-LUC reporter construct was cotransfected into HeLa cells, along with the lacZ expression vector (100 ng) and expression vectors for HR/SET1, HR/SET1m1, and HR/SET1Δ, as indicated. Closed and shaded boxes indicate the absence and presence of 0.1 μM of 9-cis-retinoic acid (RA), respectively. Normalized luciferase expressions from triplicate samples were calculated relative to that of the lacZ expressions. Similar results were obtained with CV-1 cells (data not shown). (B) GST pull-down experiments were done as previously described (17, 19) with GST alone or a GST fusion to the N-terminal 57 residues of human H3, and 20% of the total reaction mixture was loaded as input.