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SIR2α expression is widespread throughout adult tissues. RNA was isolated from a variety of tissues from wild-type mice and subjected to electrophoresis, blotting, and hybridization to cDNA probes specific for sir2α (upper panel) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, lower panel). The sir2α transcript was detected in all tissues examined and was particularly abundant in the testis and ovary.
