FIG. 13.

SIR2α is expressed at high level during spermatogenesis. Frozen sections of testis from wild-type mice were stained with polyclonal antibody to SIR2α (panel A) and with Hoechst 33258 to stain DNA (panel B). In the two seminiferous tubules shown, it is evident that the somatic cells of the tubules do not stain for SIR2α (arrows in panel A), while the immature spermatogenic cells stain to various extents before losing SIR2α expression in the elongate spermatocyte stage (panel A, right tubule). Bar, 50 μm.