TABLE 1.
Oligonucleotide primers used in PCR amplifications for plasmid construction
| Name | Sequencea | Directionb |
|---|---|---|
| F1 | GAACCCCGTGGATATGGTGG | fw |
| R1 | CATGTGGCACACCGATTGGC | rv |
| R2 | CTGCATCAGCAAGCTTCGAG | rv |
| 2bF | CGCGGAGTGCGAAAACTT | fw |
| 2bR | GGGCCAGCTTAAGCATCA | rv |
| dGCN | CATGCGAATTCTCTGCCGATCTTGGA | fw |
| dGCN | CTGTCGGATCCTTCGGCCTTATGCAG | rv |
| dADA2b-RI | GCATGAATTCATGACCACAATCGCGGATTT | fw |
| dADA2b-BHI | CGATGGATCCCCGACAGCTATCCAA | rv |
| dADA3F | GATCGAATTCATGAGTGCGAACCTGAAGAA | fw |
| dADA3R | TGACGAATTCTCCTGTCCTACATCTTTGGC | rv |
| dRPB4F | GCTAGGATCCCCGTGGATATGGTGGAT | fw |
| dRPB4R | CGATGTCGACTTAGTATTGTAAGCTGCGTTTAGT | rv |
| dRPB7F | GCTAGGATCCGAATATCGCTGGAGCAAGAGATT | fw |
| dRPB7R | CGATGTCGACTTAGTTGGACACCAATCCCAA | rv |
a
The gene-specific sequences are underlined.
b
fw, forward; rv, reverse.