TABLE 2.
Results of two-hybrid interaction assays with ADA2 and RPB4 fusion proteins
| Proteina | β-Galactosidase activityb | |
|---|---|---|
| DBD | AAD | |
| dAda2a | None | − |
| dAda2b | None | − |
| dRpb4 | None | − |
| yAda2 | None | − |
| hAda2a | None | − |
| dGcn5 | None | |
| None | dAda3 | − |
| None | dRpb4 | − |
| None | dRpb7 | − |
| None | yGcn5 | − |
| None | hGcn5 | − |
| hAda2a | hGcn5 | +++ |
| yAda2 | yGcn5 | +++ |
| dAda2a | hGcn5 | +++ |
| dAda2b | hGcn5 | +++ |
| dGcn5 | dAda2a | ++ |
| dGcn5 | dAda2b | ++ |
| dAda2a | yGcn5 | − |
| dAda2b | yGcn5 | − |
| dAda2a | dAda3 | − |
| dAda2b | dAda3 | +++ |
| dAda2a | dRpb4 | − |
| dAda2b | dRpb4 | − |
| dRpb4 | dRpb7 | ++ |
a
DBD and AAD indicate DNA-binding and activation domain fusions, respectively.
b
β-Gal activities were estimated on 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal) plates and are expressed as follows: −, no activity; ++, intermediate activity; +++, strong activity.