FIG. 4.

Immunodepletion of Mediator from HNEs causes a modest reduction in basal transcription. Basal transcription with naked DNA and chromatin templates. The plasmid template pERE, either as naked DNA (left) or assembled into chromatin (right), was transcribed with control or Mediator-depleted HNEs, as indicated. The resulting RNA products were analyzed by primer extension. Note that the chromatin experiment represents a fourfold-longer exposure than the other chromatin transcriptions shown herein, so that the low levels of basal transcription with chromatin can be observed more readily.