FIG. 6.

NF-κB p65 transcriptional activity with chromatin templates is impaired in Mediator-depleted nuclear extracts. Analysis of NF-κB p65-dependent transcription was performed with chromatin templates by using control and Mediator-depleted HNEs. The MCP-1 promoter/enhancer construct pGLM-ENH was assembled into chromatin in the presence of purified recombinant NF-κB p65 as indicated. The chromatin samples were subjected to in vitro transcription analysis, and the resulting RNA products were analyzed by primer extension. The relative transcription for each condition is indicated.