FIG. 1.
Temperature sensitivity and molecular characterization of snr1E1. (A) Percent viability of snr1E1/snr1E1 and snr1E1/+ sibling flies at different incubation temperatures. (B) Western blot of pupal-stage extracts (100 μg). SNR1 (lanes 1 and 3), SNR1E1 (lanes 2 and 4), and SNR1R3 (lane 5) at 18°C (lanes 1 and 2) and 29°C (lanes 3 to 5) were probed with anti-SNR1 antibodies. The SNR1E1 protein displayed a reduced mobility relative to that of the wild type. (C) SNR1 protein structure. Conserved domains include repeat 1, repeat 2, and a coiled-coil region (CC). The dashed line indicates the intron location. The amino acid similarity between SNR1 and INI1 is indicated below the diagram. Boxed is an alignment of repeat 2 from SNF5-family proteins, including SNR1 (D. melanogaster), INI1 (Homo sapiens), CeSNF5 (Caenorhabditis elegans), and SNF5 and SFH1 (S. cerevisiae). Shown are the known positions of affected repeat 2 residues in SNR1E1 (G256D) and mutations in INI1 (S284L), SNF5 (E582K), and SFH1 (D317K).