FIG. 7.
SNR1E1 stably associates with components of the Brm complex. (A) Immunoprecipitation (IP) with affinity-purified anti-SNR1 antibodies (C. B. Zraly et al., submitted for publication) demonstrates association with BRM and OSA in wild-type embryo extracts. Lanes: −Ab, G-Sepharose control with no primary antibody (lane represents 50% of pelleted material); E, 100 μg of extract; S, supernatant fraction (lane represents 20% of input protein extract); P, pellet fraction (lane represents 50% of coimmunoprecipitated proteins). Western blots were probed with affinity-purified rabbit anti-BRM (77), mouse monoclonal anti-OSA (15), or rat anti-SNR1 (23). (B) Immunoprecipitation (IP) with anti-SNR1 from extracts prepared with wild-type or snr1E1 homozygous pupae raised at 29°C. Lanes are as indicated for panel A.